Effect of Sintering Time of CaTiO3-CaCO3 in Osteoblastic Response of KUSA/A1 Cells

ANDREA P. RODRIGUEZ; SANCHEZ MARIA ALEJANDRA; MIHO INOUE; BETIANA FELICE; HITOSHI NAGATSUKA; ROSSANA E. MADRID; CARMELO FELICE ; HIDETSUGU TSUJIGIWA

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Lugar: Entre Ríos; Año: 2015 vol. 49 p. 159 - 162

1680-0737

In recent years, calcium titanate (CaTiO3) and carbon-containing materials have gained much attention in a number of biomedical material researches. To maximize the advantages of both materials, we developed a novel alkoxide method to get ?calcium titanate with calcium carbonate? (CaTiO3-CaCO3). Thus, our previous result indicated that calcium carbonate could play a key role in the cell response of CaTiO3 material. In the present study we evaluated the effect of sintering time of CaTiO3-CaCO3 in osteoblastic response of KUSA/A1 cells. We examined the material characterization as well as cellular proliferation and mineralization of KUSA/A1 cells cultured with the materials. The results showed that Ca-TiO3-CaCO3 material sintered during 30 min, has better influ-ence on the bone marrow stromal cells for their proliferation and mineralization compared to CaTiO3-CaCO3 sintered at 2 and 10 h. These results suggest that sintering time could be directly related to the amount of carbon within CaCO3 being crucial in cell response. In conclusion, our preliminary find-ings indicate that CaTiO3-CaCO3 could play a more dominant and favorable effect on cell response when sintered for 30 minutes.