The arabidopsis DNA polymerase Delta has a role in the deposition of transcriptionally active epigenetic marks, development and flowering

IGLESIAS, FRANCISCO; BRUERA, NATALIA; DERGAN-DYLON, S.; MARINO-BUSLJE, CRISTINA; LORENZI, H.; MATEOS, J. L.; TURCK, FRANZISKA; COUPLAND, G; CERDÁN, P.D

PLOS GENETICS

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2015 vol. 11

1553-7390

DNA replication is a key process in living organisms. DNA polymerase alfa (Pol Alfa) initiates strand synthesis, which is performed by Pol Epsilon and Pol Delta in leading and lagging strands, respectively. Whereas loss of DNA polymerase activity is incompatible with life, viable mutants of Pol Alfa  and Pol Epsilon were isolated, allowing the identification of their functions beyond DNA replication. In contrast, no viable mutants in the Pol Delta polymerase-domain were reported in multicellular organisms. Here we identify such a mutant which is also thermosensitive. Mutant plants were unable to complete development at 28°C, looked normal at 18°C, but displayed increased expression of DNA replication-stress marker genes, homologous recombination and lysine 4 histone 3 trimethylation at the SEPALLATA3 (SEP3) locus at 24° C, which correlated with ectopic expression of SEP3. Surprisingly, high expression of SEP3 in vascular tissue promoted FLOWERING LOCUS T (FT) expression, forming a positive feedback loop with SEP3 and leading to early flowering and curly leaves phenotypes. These results strongly suggest that the DNA polymerase Delta is required for the proper establishment of transcriptionally active epigenetic marks and that its failure might affect development by affecting the epigenetic control of master genes.