Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function

MARIANELA MASIN; DANIEL KERSCHENSTEINER; KERSTIN DÜMKE; MARIA E. RUBIO; FLORENTINA SOTO

JOURNAL OF BIOLOGICAL CHEMISTRY

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Lugar: Bethesda, Maryland; Año: 2006 vol. 281 p. 4100 - 4108

0021-9258

Ionotropic receptors in the neuronal plasmamembrane are organized in macromolecular complexes, which assure their proper localization and regulate signal transduction. P2X receptors, the ionotropic receptors activated by extracellular ATP, have been shown to influence synaptic transmission. Using a yeast two-hybrid approach with the P2X2 subunit C-terminal domain as bait we isolated the beta-amyloid precursor protein-binding proteins Fe65 and Fe65-like 1 as the first identified proteins interacting with neuronal P2X receptors.Weconfirmed the direct interaction of Fe65 and the P2X2 C-terminal domain by glutathione S-transferase pull-down experiments. No interaction was observed between Fe65 and the naturally occurring P2X2 splice variant P2X2(b), indicating that alternative splicing can regulate the receptor complex assembly.We generated two antibodies to Fe65 to determine its subcellular localization using postembedding immunogold labeling electronmicroscopy. We found labeling for Fe65 at the pre- and postsynaptic specialization of CA1 hippocampal pyramidal cell/Schaffer collateral synapses. By double immunogold labeling, we determined that Fe65 colocalizes with P2X2 subunits at the postsynaptic specializationof excitatory synapses. Moreover, P2X2 and Fe65 could be coimmunoprecipitated from brain membrane extracts, demonstrating that the interaction occurs in vivo. The assembly with Fe65 regulates the functional properties of P2X2 receptors. Thus, the time- and activation-dependent change in ionic selectivity of P2X2 receptors was inhibited by coexpression of Fe65, suggesting a novel role for Fe65 in regulating P2X receptor function and ATP-mediated synaptic transmission.