Potentiation of the probiotic effect of Saccharomyces cerevisiae RC016 strain with Minthostachys verticillata essential oil

ESCOBAR FM; SABINI MC; CARIDDI LN; BAGNIS G; SOLTERMAN A; PEREYRA C; MAGNOLI A; CAVAGLIERI L

Congreso; XXXIII Jornadas Científicas Asociación de Biología de Tucumán; 2016

This study potentiates the effect of a S. cerevisiae RC016 strain against aflatoxin B1 (AFB1) damage when the strain was included in the rat diet with Minthostachys verticillata essential oil (Mv-EO). The probiotic and Mv-EO were orally administered to Wistar rats. Animal were assigned to eight groups (n = 5): feed, probiotic, Mv-EO and probiotic + Mv-EO controls, one level of AFB1-contaminated feed (40 μg/kg) and three treatments including the probiotic and the toxin; Mv-EO and AFB1; and the probiotic, Mv-EO and the toxin. Food and water were available ad libitum throughout the experimental period (45 days). There was no mortality, adverse effects on general conditions or changes in body weight, food consumption or feed conversion efficiency throughout the study. However, rats administered the probiotic and Mv-EO exhibited 10% increase in body weight in comparison with other groups. Subchronic administration of probiotic and Mv-EO did not alter the weight, morphological or histopathological analyses of liver and kidney. Genotoxicity was tested by micronucleus on bone marrow cells. Rats treated with AFB1 showed a significant increase in micronucleated erythrocytes (MNE) compared to other groups. However, rats treated with the probiotic and/or MV-EO decreased the genotoxic damage caused by the toxin; a similar average value of MNE compared to the negative control group was observed. Conclusion: Incorporation of MV-EO enhances the probiotic effect of S. cerevisiae RC016 strain in the formulation of feed additives and increases animal productivity.