Protective effect of chlorogenic acid against genotoxic damage induced by ochratoxin a in rats.

CAMPRA NA; CARIDDI LN; ESCOBAR FM; SABINI MC; MAÑAS F; SABINI L; DALCERO A

Congreso; XXXIII Jornadas Científicas Asociación de Biología de Tucumán; 2016

Ochratoxin A (OTA) is a mycotoxin with nephrotoxic, genotoxic and immunosuppressant properties. Some research indicates that the damage caused by OTA in the kidney may be associated with carcinogenic potential. In a previous study we confirmed that OTA induced DNA damage in mice bone marrow cells and that chlorogenic acid (ChlA) exerted a protective effect on this action but not through an antioxidant mechanism. The aim of this study was to characterize in vivo the ability of ChlA to reverse the genotoxic effects induced by OTA in a subacute toxicity test. Wistar rats were fed orally for 28 days with OTA (0.4 mg/kg), ChlA (5 mg/kg) or a combination of OTA (0.4 mg/kg)+ChlA(5 mg/kg). As control vehicle PBS/DMSO (0.03%) was used. The micronucleus assay on bone marrow and comet assay on blood were performed. Increase in micronuclei (MN) in polychromatic erythrocytes (PCE) from OTA-treated animals was observed (p