Capacity of mayoritary compounds of Achyrocline satureioides to protect from the damage induced by aflatoxin b1 in Wistar rats

SABINI MC; ESCOBAR FM; CARIDDI LN; BAGNIS G; COMINI L; MENIS CANDELA F; MAGNOLI A

Congreso; XLVIII Reunión anual de la Sociedad Argentina de Farmacología Experimental (SAFE); 2016

Aflatoxin B1 (AFB1), produced by A. flavus and A. parasiticus, is carcinogenic, teratogenic, hepatotoxic, immunotoxic. It is the most important worldwide mycotoxin for its effect on public health and animal productivity. Macela has several scientifically proven medicinal properties, such as antioxidant, antimicrobial. The objective was to evaluate the ability of majority compounds(MC) of A. satureioides to protect of genotoxic and liver damage induced by AFB1 in rats. Wistar rats (200 g) in lots of 4 (2 males and 2 females) were used. Animals were inoculated by intraperitoneal injection. Treatments were: 1-AFB1 (1 mg/kg body weight) + Luteolin (L) (2.5 mg/ kg b.w.); 2-AFB1 (1 mg/kg) + Quercetin (Q) (2.5 mg/kg); 3-AFB1 (1 mg/kg) + Chlorogenic acid (CHLA) (5 mg/kg); 4-Negative control: saline solution; 5-Positive control: cyclophosphamide 30 mg/kg; 6-AFB1 Control: AFB1 (1 mg/kg) dissolved in methanol (5%) and saline solution. Animals were sacrificed by cervical dislocation at 24h post-injection. Experiments: 1-Micronuclei in mouse bone marrow: Schmidt W. (1975), number of micronuclei in 1000 polychromatic erythrocytes (PCE) and toxicity index were determined. 2- Hystopathology: Parts of the organs were pre-served in 10% buffered formaldehyde (pH 7.4). These samples were cut at 4 μm thickness and subjected to haematoxylin/eosin staining for microscopic histological. Photomicrographs were taken and analyzed. The results of genotoxicity revealed that AFB1 at 1 mg/kg showed an index of genotoxicity of 35 MN/1000 PCE, showing statistically significant difference with the negative control (p