Evaluation of the adjuvant effect of Minthostachys verticillata essential oil for its possible use in vaccines

CAMPRA NA; RAVIOLO J; REINOSO E; CARIDDI LN

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

The aim of this study was to determine the effect of essential oil (EO) as adjuvant evaluating the humoral and cellular immune response. Balb/c mice (n=4 per group) were immunized by subcutaneous injection (day 0) and revaccinated (at day 14 and 28) as follows: Group 1:100 μl PBS; Group 2:100 μl ovalbumin (OVA) 0.2 mg/ml; Group 3: OVA + Al(OH)3 0.5 mg/ml; Group 4-6: OVA + EO (1.25; 2.5; 5 mg/ml); Group 7: OVA + Al(OH)3 + EO (2.5 mg/ml). Seven days after revaccination, serum samples were collected and analyzed for antigen-specific total antibodies (IgG, IgM and IgA) by indirect ELISA. For delayed-type hypersensitivity (DTH) test, mice (n=2 per group) were immunized by subcutaneous injection on days 0 and 14 as described for groups 1, 2, 3, 5 and 7. Seven days after immunization a challenge with OVA in the left footpads were performed by intradermal injection. The thickness of hind footpads was measured before and 24 and 48 h after the OVA injection. Results were expressed as the difference in thickness of footpads after and before the inoculation. Any animal died, no clinical signs were observed in the inoculation area either generalized reactions in any group. A significant increase in antibodies (Ab) levels was observed in the groups 3, 5, 6 and 7 respect to group 2 (p