Modulator effects of the essential oil of Minthostachys verticillata on macrophage functions

MONTIRONI I; DECOTE-RICARDO D; LA ROQUE DE FREITAS IF; FREIRE-DE-LIMA C; REINOSO E; CARIDDI LN

Congreso; LXIV Reunión anual de la Sociedad Argentina de Inmunología (SAI).; 2016

Minthostachys verticillata is an Argentinean medicinal plant and its essential oil showed immunostimulatory activity on human T and B cells and anti inflammatory properties. The aim of this study was to characterize the effect of essential oil on macrophage phagocytosis. RAW 264.7 line was used. Cells were exposed to different concentrations of essential oil (EO) (0, 0.5, 5, 50, 60and 75 μg/ml) at different times in order to evaluate the effect on cell proliferation, adherence and phagocytosis. For assess adhesion and phagocytosis, a suspension of yeast Saccharomyces cerevisiae was added to each well in a 10:1 relation. Cells with only D-MEM and DMSO 0.1% in D-MEM complete were used ascontrol. The results showed that EO treatment did not induce cell proliferation. A significant dose-dependent increase was observed in the number of macrophages that adhered yeast (p