Cholorogenic acid protects against toxic damage induced by ochratoxin A in a 28-day oral treatment in rats.

CARIDDI LN; CAMPRA NA; ESCOBAR FM; SABINI MC; BAGNIS G; ZANON S; SABINI LI; DALCERO A

Congreso; III Reunión Conjunta de Sociedades de Biología de la República Argentina; 2015

Ochratoxin A (OTA) is a mycotoxin with nephrotoxic, genotoxic and immunosuppressive properties. Recent studies showed that treatment for 24 h with polyphenols (chlorogenic acid, caffeic acid and luteolin) reduced the toxic effects of OTA in vitro and in vivo, chlorogenic acid (ChlA) being the compound with better effects. The aim of this study was to characterize in vivo ChlA capacity to reverse the toxic effects induced by OTA by a subacute toxicity test. Wistar rats were fed orally for 28 days with OTA (0.4 mg/kg), ChlA (5 mg/kg), or combinations OTA (0.4 mg/kg) + ChlA (5 mg/kg). PBS/methanol (0.03%) was used as vehicle control. No deaths or decrease in food intake or body weight were recorded in any experimental groups. The control group and the animals treated with ChlA alone showed no changes in any of the parameters evaluated. In animals treated with OTA significant changes were observed such as decrease in urine volume, decrease in urine creatinine values, proteinuria, occult blood, and decrease in absolute and relative kidney weight and histopathological lesions characteristic of kidney damage. The group treated with the combination OTA + ChlA was similar to the negative control group in the evaluation of certain parameters of toxicity, which would indicate a protective effect of ChlA on renal damage induced by OTA. These results provide the basis for further studies related to the protective effects of polyphenols and the mechanism of action exerted on the toxicity of OTA, considering that similar effects could occur by administration of these compounds in the diet of animal species susceptible to OTA.