Characterization of the effect of dose and exposure time of Minthostachys verticillata (Griseb.) Epling essential oil on cells of bovine and murine mammary gland

MONTIRONI I; RAVIOLO J; BOHL L; CAMPRA NA; BAGNIS G; PORPORATTO C; REINOSO E; CARIDDI LN

Congreso; III Reunión Conjunta de Sociedades de Biología de la República Argentina; 2015

Previous studies demonstrated that the essential oil (AE) obtained from Minthostachysverticillata has immunostimulatory activity on human T and B cells. The aim of this study was to characterize the cytotoxic effect of AE on mammary gland cells (GM), both in vitro and invivo, to evaluate their possible application as an immunomodulator in intramammary infections. For this, the effect of dose and exposure time of AE was assessed in a line of bovine mammary epithelial cells (MAC-T) and GM of Balb/c female mice in the breastfeeding period. The MAC-T cells were exposed to different concentrations of AE (0, 10, 25, 50, 100, 250 and 500 μg/mL) for 6, 12, 24 and 48 h and viability was determined by MTT assay .The female mice were divided into 4 groups (6 mice per group) and inoculated into the right (R4) and left (L4) abdominal GM. Group 1 (control) received 100 μL of PBS/DMSO (0.03%) while groups 2, 3 and 4 received 100 μLof AE (25, 50 and 100 ug / ml). Animals were sacrificed at 24, 48 and 72 h post-inoculation and the GM were removed and processed according to routine histological methods. AE did not alter the viability of the MAC-T cells independently of the tested concentration and exposure time. The histopathology of the treated GM with AE revealed no injuries or signs of inflammation at any of the tested concentrations or times. The results encourage the study of the immunomodulatory effects of AE in mouse GM and provide the basis for future studies in a bovine model.