Toxicological evaluation of cold aqueous extract of Achyrocline satureioides

SABINI MC; ESCOBAR FM; CARIDDI LN; ACOSTA A; MAÑAS F; SUTIL SB; SABINI LI

Congreso; XXX Reunión Científica Anual Sociedad de Biología de Cuyo; 2012

Achyrocline satureioides Lam. (DC) is used in traditional medicine. This species has several bioactive properties such as antiviral activity. Previously, we reported absence of genotoxicity by Allium cepa L test. However, the genetic toxicology requires more of one study to determine genotoxic effects of a substance in human. The aim of this study was to determine the genotoxic ability of cold aqueous extract (CAE) of A. satureioides by micronucleus test (MN) in mouse bone marrow and comet assay (CA). Comet assay was determined on Vero cells. Five concentrations of CAE (10, 25, 50, 250 and 500 µg/ml) were employed. Negative and positive cell controls were treated with MEM and Mitomicina C (0,011 mM), respectively. For MN assay were employed 7 groups of Balb-C mice. CAE (0.2 ml of 50, 100 and 250 mg kg-1) was administrated by intraperitoneal injection. Negative and positive controls were inoculated with saline and cyclophosphamide (20 mg kg-1), respectively. No genotoxic activity was detected by means of MN assay, neither by CA at 50µg/ml of CAE or lower. This absence of genotoxicity, added to the relevant antiviral activity, could justify the use of CAE of A. satureioides in treatments of those pathologies; the results suggest its sure use in folk medicine.