Minthostachys verticillata: activity on allergenic fungi and mitogenic effects on B cells

CARIDDI LN; GAMBERO ML; USORACH M; DALCERO A

Villa Giardino, Cordoba

Jornada; XV Jornadas Cientificas de la Sociedad de Biologia de Cordoba; 2005

Essential oil (EO) and decoction (D) from Minthostachys verticillata (Griseb) Epling (Mv) have antibacterial and antiviral activities. In cyto-morphological studies it was observed that Mv derivates has mitogenic properties such as PHA with CD 8 (+) lymphocytes expansion. Moreover, D and EO showed ability to inhibit B-hexosaminidase released from allergic patient?s basophils. In this study the mitogenic ability of D and EO derived from Mv compared to PWM. B cells by IF were evaluated. Environmental fungi were isolated and identified and D from Mv anti-fungic effects was investigated. Lymphocytes from 15 allergic patients hypersensitive to environmental fungi and l0 healthy individuals were cultured. D and EO were obtained from leaves and stems. Lymphocytes isolation was done by density gradient and lymphocyte proliferation was evaluated by MTT colorimetric assay. The B cells were determined by IF. Most fungi genus were isolated and identified from the houses patients. Different concentrations were used to show anti-fungic activity. DMSO was used as negative control and miconazole or clotrimazole as positive controls. The lymphocyte proliferation was stimulated by D: 0,7 mg/ml and EO: 0,16 mg/mI, in similar levels to PWM (p=NS). Old PI were l,30. The 54% of D stimulated cells were LB. Penicillium, sp. Aspergillus sp. Cladosporium sp and Alternaria sp were the main genus isolated. D (0.625 ug/ml) and (10 ug/ml; 2,5 ug/mI y 1,25 ug/ml) inhibited respectively Aspergillus sp and Alternaria sp. D vs controls difference was: p