Achyrocline satureioides (Lam) DC: cytotoxic capacity against lymphocytes of equines and chemical analysis of its cold aqueous extract

SABINI MC; CARIDDI LN; ESCOBAR FM; COMINI L; TORRES C; NUÑEZ MONTOYA S; SERENO J; CANTERO J; SABINI L

Congreso; XIX Jornadas Científicas, Sociedad de Biología de Córdoba; 2013

Introduction: Achyrocline satureioides (Lam.) DC is an important species of Asteraceae family, is commonly known as "marcela del campo". Previous studies demonstrated the ability of cold aqueous extract (CAE) to inhibit the Western Equine Encephalitis virus. It is necessary to evaluate the cytotoxic effect of CAE on normal cells of equines to determine its safety and establish a selectivity index. Objective: To evaluate the cytotoxicity on lymphocytes of equines and analyze the chemical composition of CAE of A. satureioides. Methodology: Vegetal aerial parts were submitted to extraction with cold (4ºC) water for two days. Then, the solution was filtered and lyophilized to obtain the CAE. Equine lymphocyte cultures were treated with different concentrations (0-3000 µg/mL) of CAE, for 18-24 h. Then cytotoxicity was evaluated by two techniques: a) the staining by Trypan Blue Exclusion (TBE) and b) Reduction of MTT. Analysis of chemical composition was performed by HPLC-ESI-MS/MS. Results and Discussion: Cytotoxic studies showed low toxicity of CAE. It was demonstrated dose-response relationship on lymphocytes equine by both TBE (CC50=1300 µg/mL) and MTT (CC50=700 µg/mL). Chemical analysis indicated the presence of quercetin-3-O methylquercetin and luteolin.