Evaluation of cytotoxic effects of metabolites identified in aqueous extract of Baccharis articulata for its possible application as bioprotectors in mycotoxicosis

CARIDDI LN; SABINI MC; ESCOBAR FM; MONTIRONI I; COMINI L; TORRES C; CAVAGLIERI L; SABINI L; DALCERO A

Congreso; XIX Jornadas Científicas, Sociedad de Biología de Córdoba; 2013

Ochratoxin A (OTA) is a dangerous mycotoxin in food and feed. Pure compounds with antioxidant properties are effective for reducing the toxic effects of mycotoxins. A previous study identified the major compounds of the aqueous extract of B. articulata, a medicinal plant (luteolin, acacetin and chlorogenic acid). The aim of this study was to determine the in vitro cytotoxic effect of these compounds.HPLC analysis of the extract revealed the presence of luteolin (L) (1.96 ± 0.27%), acacetin (A) (1.12 ± 0.14%) and chlorogenic acid (CA) (0.29 ± 0.05%). Lymphocytes were obtained from Wistar rats and exposed to L, A and AC (10, 50, 100 and 200μg/mL) (n = 3) in independent trials and in combinations (L + A, L + AC, AC and A + L + A + AC) for 18-24 h. Cells with RPMI- 1640 alone were used as control. Toxicity was evaluated by MTT reduction. Test compounds alone or in combination did not affect the cell viability (L: 96,50 ± 1.28%, A: 97,70 ± 0.98%, AC: 97,82 ± 1.08%, L + A: 96,56 ± 1.00%, L + AC: 98,02 ± 0,76, A + AC: 96,87 ± 1.33%, L + A + AC: 97,78 ± 1.37%) compared to control (98.70 ± 0.88%). The results encourage evaluation of these compounds to neutralize the toxic effects of OTA.