Minthostachys verticillata essential oil modulates innate immune response in bovine epithelial cells challenged with Staphylococcus aureus vía NFκB

ARSAUTE S; MONTIRONI ID; CECCHINI ME; REINOSO E; CARIDDI LN

Congreso; LXXI Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2023

In a previous study we demonstrated the ability of essential oil (EO) of Minthostachys verticillata, an Argentinian plant, to modulate the IL-1β and IL-6 synthesis in bovine epithelial cells (MAC-T), in the presence or absence of an inactivated Staphylococcus aureus strain (iSa), isolated from cows with mastitis. In cells pre-treated with EO and then challenged with iSa, we observed a decrease in both cytokines’ levels between 24 and 48 h post-challenge. Therefore, the aim of this work was to evaluate the effect of EO on the synthesis of anti-inflammatory cytokines as well as to determine the relative expression of NFκ-B transcription factor and TLR2 receptor in MAC-T cells, in the presence or absence of iSa. The cells were treated with EO (25, 50 and 100 µg/mL), iSa (5x106 CFU/mL) and pretreated with EO and then challenged with iSa, at different times (2, 6, 24 and 48 h). Cells without stimulation were used as control. IL-4 levels were quantified in the cell supernatant by sandwich ELISA. The mRNA relative expression of NFκ-B and TLR2 was measured by qPCR. As expected, in cells stimulated with iSa, IL-4 values were similar to those found in control cells. In cells stimulated with EO at three concentrations, the IL-4 values were significantly higher than those observed in control cells at 2, 6 and 24 h (p