Minthostachys verticillata essential oil modulates IL-1β AND IL-6 synthesis in bovine epithelial cells challenged with a Staphylococcus aureus strain

ARSAUTE S; MONTIRONI ID; CECCHINI ME; REINOSO E; CARIDDI LN

Congreso; Reunión conjunta SAIC SAI&FAIC SAFIS; 2022

In previous studies we showed that the essential oil (EO) of Minthostachys verticillata, a native plant species, has the ability to modulate the immune response. The aim of this work was to evaluate the effect of EO on the synthesis of proinflammatory cytokines in bovine mammary gland epithelial cells (MAC-T) in the presence or absence of a Staphylococcus aureus strain (Sa) isolated from cows with mastitis. The cells were treated with EO (25, 50 and 100 µg/mL), Sa (5x106 CFU/mL) and pretreated with EO and then challenged with Sa at different times (2, 6, 24 and 48 h). Cells without stimulation were used as control. IL-1β and IL-6 cytokines were quantified in the cell supernatant by sandwich ELISA. MAC-T cells stimulated with Sa produced higher levels of IL-1β and IL-6 than untreated cells (p