Nanoencapsulated essential oil modulates immunological and oxidative parameters in weaned pigs

MONTIRONI ID; ROMA D; ARSAUTE S; CECCHINI ME; PEDRAZA ML; PINOTTI A; MAÑAS F; BESSONE F; ALUSTIZA F; BELLINGERI R; CARIDDI LN

Mar del Plata

Congreso; Reunión conjunta SAIC SAI&FAIC SAFIS; 2022

In a previous study, we demonstrated that Minthostachys verticillata essential oil (EO) modulated mucosal and systemic immune response in a mouse model. The aim of this work was to evaluate immunomodulatory effect of nanoemulsion based on M. verticillata EO (NEO) in weaned pigs. NEO was developed with EO (20%v/v), Tween 80 (0.75%v/v), Span 60 (0.25%w/v) and water (79%v/v) and was synthesized by the high-energy method. Biocompatibility studies on Caco-2 cells showed that NEO was not toxic up to 500 μg/ml. NEO immunomodulatory effect was evaluated in male weaned pigs (n=36) divided into six groups (n=6) that received orally for 30 consecutive days the followed: Group 1: Control (ClNa 0.9%), Group 2: Vehicle control (Tween 80, Span 60 and water), Group 3: EO (10 mg/Kg/day), Groups 4-6: NEO (2.5, 5 or 10 mg/Kg/day). An increase in body weight gain was observed in EO (10 mg/Kg/day) and NEO (10 mg/Kg/day) groups compared to control (p