CONICET | Buscador de Institutos y Recursos Humanos

RESUMEN Los mit¨®genos PHA y ConA son capaces de estimular a los linfocitos T CD3+, incluidos los CD4+ y CD8+ (¦Á/¦Â ¦Ã/¦Ä) conduciendo a su activaci¨®n policlonal. Minthostachys verticillata (Griseb.) Epling, con nombre vulgar "peperina", es un subarbusto arom¨¢tico que abunda en el centro y noroeste de Argentina. Su aceite esencial ha demostrado in vitro capacidades antial¨¦rgicas y linfoproliferativas. En este trabajo se investigaron in vitro los efectos de los compuestos puros: pulegona, mentona y limoneno, aislados del aceite esencial de M. verticillata, como activadores policlonales de linfocitos humanos. Las c¨¦lulas, aisladas de muestras de sangre de individuos sanos, fueron cultivadas y estimuladas con PHA, ConA, pulegona, mentona o limoneno, en ensayos independientes. Adem¨¢s se realizaron cultivos co-estimulados con: pulegona/mentona, pulegona/limoneno, mentona/limoneno y pulegona/mentona/limoneno en porcentajes similares a los que se encuentran estos compuestos en el vegetal (63% de pulegona, 16% de mentona y 2% de limoneno). Los cultivos se realizaron aplicando el m¨¦todo colorim¨¦trico del MTT. La combinaci¨®n de los tres compuestos puros logr¨® una expansi¨®n clonal de los linfocitos similar a la de PHA o ConA (p=ns). Estos resultados revelaron la capacidad mitog¨¦nica de la combinaci¨®n de los tres compuestos puros pudiendo los mismos ser utilizados in vitro para evaluar la funcionalidad de la inmunidad celular ¦Á/¦Â ¦Ã/¦Ä) conduciendo a su activaci¨®n policlonal. Minthostachys verticillata (Griseb.) Epling, con nombre vulgar "peperina", es un subarbusto arom¨¢tico que abunda en el centro y noroeste de Argentina. Su aceite esencial ha demostrado in vitro capacidades antial¨¦rgicas y linfoproliferativas. En este trabajo se investigaron in vitro los efectos de los compuestos puros: pulegona, mentona y limoneno, aislados del aceite esencial de M. verticillata, como activadores policlonales de linfocitos humanos. Las c¨¦lulas, aisladas de muestras de sangre de individuos sanos, fueron cultivadas y estimuladas con PHA, ConA, pulegona, mentona o limoneno, en ensayos independientes. Adem¨¢s se realizaron cultivos co-estimulados con: pulegona/mentona, pulegona/limoneno, mentona/limoneno y pulegona/mentona/limoneno en porcentajes similares a los que se encuentran estos compuestos en el vegetal (63% de pulegona, 16% de mentona y 2% de limoneno). Los cultivos se realizaron aplicando el m¨¦todo colorim¨¦trico del MTT. La combinaci¨®n de los tres compuestos puros logr¨® una expansi¨®n clonal de los linfocitos similar a la de PHA o ConA (p=ns). Estos resultados revelaron la capacidad mitog¨¦nica de la combinaci¨®n de los tres compuestos puros pudiendo los mismos ser utilizados in vitro para evaluar la funcionalidad de la inmunidad celular Los mit¨®genos PHA y ConA son capaces de estimular a los linfocitos T CD3+, incluidos los CD4+ y CD8+ (¦Á/¦Â ¦Ã/¦Ä) conduciendo a su activaci¨®n policlonal. Minthostachys verticillata (Griseb.) Epling, con nombre vulgar "peperina", es un subarbusto arom¨¢tico que abunda en el centro y noroeste de Argentina. Su aceite esencial ha demostrado in vitro capacidades antial¨¦rgicas y linfoproliferativas. En este trabajo se investigaron in vitro los efectos de los compuestos puros: pulegona, mentona y limoneno, aislados del aceite esencial de M. verticillata, como activadores policlonales de linfocitos humanos. Las c¨¦lulas, aisladas de muestras de sangre de individuos sanos, fueron cultivadas y estimuladas con PHA, ConA, pulegona, mentona o limoneno, en ensayos independientes. Adem¨¢s se realizaron cultivos co-estimulados con: pulegona/mentona, pulegona/limoneno, mentona/limoneno y pulegona/mentona/limoneno en porcentajes similares a los que se encuentran estos compuestos en el vegetal (63% de pulegona, 16% de mentona y 2% de limoneno). Los cultivos se realizaron aplicando el m¨¦todo colorim¨¦trico del MTT. La combinaci¨®n de los tres compuestos puros logr¨® una expansi¨®n clonal de los linfocitos similar a la de PHA o ConA (p=ns). Estos resultados revelaron la capacidad mitog¨¦nica de la combinaci¨®n de los tres compuestos puros pudiendo los mismos ser utilizados in vitro para evaluar la funcionalidad de la inmunidad celular ¦Á/¦Â ¦Ã/¦Ä) conduciendo a su activaci¨®n policlonal. Minthostachys verticillata (Griseb.) Epling, con nombre vulgar "peperina", es un subarbusto arom¨¢tico que abunda en el centro y noroeste de Argentina. Su aceite esencial ha demostrado in vitro capacidades antial¨¦rgicas y linfoproliferativas. En este trabajo se investigaron in vitro los efectos de los compuestos puros: pulegona, mentona y limoneno, aislados del aceite esencial de M. verticillata, como activadores policlonales de linfocitos humanos. Las c¨¦lulas, aisladas de muestras de sangre de individuos sanos, fueron cultivadas y estimuladas con PHA, ConA, pulegona, mentona o limoneno, en ensayos independientes. Adem¨¢s se realizaron cultivos co-estimulados con: pulegona/mentona, pulegona/limoneno, mentona/limoneno y pulegona/mentona/limoneno en porcentajes similares a los que se encuentran estos compuestos en el vegetal (63% de pulegona, 16% de mentona y 2% de limoneno). Los cultivos se realizaron aplicando el m¨¦todo colorim¨¦trico del MTT. La combinaci¨®n de los tres compuestos puros logr¨® una expansi¨®n clonal de los linfocitos similar a la de PHA o ConA (p=ns). Estos resultados revelaron la capacidad mitog¨¦nica de la combinaci¨®n de los tres compuestos puros pudiendo los mismos ser utilizados in vitro para evaluar la funcionalidad de la inmunidad celular ABSTRACT The PHA and ConA mitogens are able to stimulate to CD3+ T cells included (¦Á/¦Â ¦Ã/¦Ä) CD4+ and CD8+ T cells leading to its polyclonal activation. Minthostachys verticillata (Griseb.) Epling commonly known as "peperina" is an aromatic bush that grow in the north-west and central regions of Argentina. Its essential oil has demonstrated anti-allergic and lymphoproliferative properties in vitro. In this work, the in vitro effects of pure compounds isolated of the essential oil of M verticillata: pulegone, menthone and limonene, like polyclonals activators on human lymphocytes, were investigated. The cells, isolated from peripheral blood, were cultured in independent assay and stimulated with PHA, ConA, pulegone, menthone or limonene. In addition co-stimulated cultures with: pulegone/men-thone, pulegone/limonene, menthone/limonene and pulegone/menthone/limonene in per-centtage similar to which is these compounds in the vegetable (63% of pulegone, 16% of menthone and 2% of limonene) were realized. The cultures were making by MTT colorimetric assay. The combination of three pure compounds was effective to clonal expansion of lymphocytes like PHA o ConA (p=ns). These results revealed the mitogenic capacity of the combination of three pure compounds such being able to be used in vitro to evaluate the functionality of the cellular immunity. ¦Á/¦Â ¦Ã/¦Ä) CD4+ and CD8+ T cells leading to its polyclonal activation. Minthostachys verticillata (Griseb.) Epling commonly known as "peperina" is an aromatic bush that grow in the north-west and central regions of Argentina. Its essential oil has demonstrated anti-allergic and lymphoproliferative properties in vitro. In this work, the in vitro effects of pure compounds isolated of the essential oil of M verticillata: pulegone, menthone and limonene, like polyclonals activators on human lymphocytes, were investigated. The cells, isolated from peripheral blood, were cultured in independent assay and stimulated with PHA, ConA, pulegone, menthone or limonene. In addition co-stimulated cultures with: pulegone/men-thone, pulegone/limonene, menthone/limonene and pulegone/menthone/limonene in per-centtage similar to which is these compounds in the vegetable (63% of pulegone, 16% of menthone and 2% of limonene) were realized. The cultures were making by MTT colorimetric assay. The combination of three pure compounds was effective to clonal expansion of lymphocytes like PHA o ConA (p=ns). These results revealed the mitogenic capacity of the combination of three pure compounds such being able to be used in vitro to evaluate the functionality of the cellular immunity. The PHA and ConA mitogens are able to stimulate to CD3+ T cells included (¦Á/¦Â ¦Ã/¦Ä) CD4+ and CD8+ T cells leading to its polyclonal activation. Minthostachys verticillata (Griseb.) Epling commonly known as "peperina" is an aromatic bush that grow in the north-west and central regions of Argentina. Its essential oil has demonstrated anti-allergic and lymphoproliferative properties in vitro. In this work, the in vitro effects of pure compounds isolated of the essential oil of M verticillata: pulegone, menthone and limonene, like polyclonals activators on human lymphocytes, were investigated. The cells, isolated from peripheral blood, were cultured in independent assay and stimulated with PHA, ConA, pulegone, menthone or limonene. In addition co-stimulated cultures with: pulegone/men-thone, pulegone/limonene, menthone/limonene and pulegone/menthone/limonene in per-centtage similar to which is these compounds in the vegetable (63% of pulegone, 16% of menthone and 2% of limonene) were realized. The cultures were making by MTT colorimetric assay. The combination of three pure compounds was effective to clonal expansion of lymphocytes like PHA o ConA (p=ns). These results revealed the mitogenic capacity of the combination of three pure compounds such being able to be used in vitro to evaluate the functionality of the cellular immunity. ¦Á/¦Â ¦Ã/¦Ä) CD4+ and CD8+ T cells leading to its polyclonal activation. Minthostachys verticillata (Griseb.) Epling commonly known as "peperina" is an aromatic bush that grow in the north-west and central regions of Argentina. Its essential oil has demonstrated anti-allergic and lymphoproliferative properties in vitro. In this work, the in vitro effects of pure compounds isolated of the essential oil of M verticillata: pulegone, menthone and limonene, like polyclonals activators on human lymphocytes, were investigated. The cells, isolated from peripheral blood, were cultured in independent assay and stimulated with PHA, ConA, pulegone, menthone or limonene. In addition co-stimulated cultures with: pulegone/men-thone, pulegone/limonene, menthone/limonene and pulegone/menthone/limonene in per-centtage similar to which is these compounds in the vegetable (63% of pulegone, 16% of menthone and 2% of limonene) were realized. The cultures were making by MTT colorimetric assay. The combination of three pure compounds was effective to clonal expansion of lymphocytes like PHA o ConA (p=ns). These results revealed the mitogenic capacity of the combination of three pure compounds such being able to be used in vitro to evaluate the functionality of the cellular immunity.

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