A single acyline treatment significantly impairs canine spermatogenesis

H. KÖRBER, A. FRIMØDT RONNOW, M.J. PAEZ LIZCANO, M. FAYA, C GOBELLO, S. GOERICKE-PESCH

REPRODUCTION IN DOMESTIC ANIMALS (1990)

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Copenhage; Año: 2018 vol. 53 p. 22 - 22

0936-6768

Effect of a single acyline treatment oncanine spermatogenesisEffekte einer einmaligen Acyline Behandlung aufcanine SpermatogeneseH Körber1; A Frimødt Ronnow1; MJ Paez Lizcano1; M Faya2,3;C Gobello2,4; S Goericke-Pesch11Section for Veterinary Reproduction and Obstetrics, University of Copenhagen,Frederiksberg, Denmark; 2National Research Council (CONICET), Cordoba,Argentina; 3Catholic University of Cordoba, Cordoba, Argentina; 4Laboratory ofReproductive Physiology, Faculty of Veterinary Medicine, National University of LaPlata (NULP), La Plata, ArgentinaThe use of GnRH antagonists is discussed as a possible nonsurgical approach to castration of male dogs. Information abouthistological effects on spermatogenesis is, however, missing.Therefore, we investigated the effect of a single GnRH antagonist treatment on spermatogenesis in canine testes. Sexually mature, healthy male dogs (n = 4) were treated subcutaneously witha single injection of the GnRH antagonist acyline (330 μg/kg) andsurgically castrated two weeks later. Five untreated normospermic dogs served as controls. From each dog, 200 cross-sections ofapproximately round tubuli seminiferi contorti were evaluated forwhether there was spermatogenic arrest or undisturbed spermatogenesis. In case of full spermatogenesis being present, the different tubules were categorized in stages (Stage I-VIII). Additionally,the area of 100 approximately round tubules from each dog wasdetermined. The investigators were blinded to the group thedogs belong to. Histological changes in the tubular structurewith a disruption of the normal cellular distribution of the testicular tissue was observed in all treated dogs with occurrenceof spermatogenic arrest being significantly higher at the level ofround (p = 0.0199) and elongating spermatids (p = 0.0108) compared to the control group. Percentage distribution of spermatogenic stages among seminiferous tubules differed significantlyfor all stages (from p = 0.0160 to p = 0.0179), except stage VIII.Furthermore, the tubular area was significantly reduced in treatedcompared to control dogs (p < 0.001). The histological changes inthe tubular structure of the testicular tissue indicate that a singleacyline treatment has a negative impact on canine spermatogenesis. (The authors thank the National Institutes of Health, USA forprovision of acyline to CG.)