CORRELATION BETWEEN BIOMARKERS OF OXIDATIVE STRESS AND DEGREE OF COGNITIVE IMPAIRMENT IN PATIENTS WITH LEUKOARAIOSIS AND PROBABLE ALZHEIMER'S DISEASE

ALEJANDRA CIMATO; MARGARITA MARTINEZ SARRASAGUE; FABIANA LAIRION; CHRISTIAN SAPORITO; JORGE SERRA; ENRIQUE MARSCHOFF; LILIANA OUDERK; GRACIELA ADA BIANCHI; RAUL DOMINGUEZ; MARISA REPETTO

Mar del Plata

Congreso; 67º Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2022

Sociedad Argentina de Investigación Clínica

Oxidative stress (OS) and oxidative damage (OD) are associated to the etiopathogenesis ofLeukoaraiosis (L) and Alzheimer disease (A). The aim of this work was to evaluatewhether OS markers in the blood of patients with L and A correlate with the degree ofcognitive impairment (CI). Methods: OS biomarkers: carbonylated proteins (CO), oxidizedlipids (TBARS), superoxide dismutase (SOD), catalase (CA), total (GSHt), reduced (GSH),and oxidized (GSSG) glutathione (by spectrophotometry), and IL-6 (by ELISA) weredetermined in the blood of elderly patients (75±6 years): 17 with a diagnosis of L (magneticresonance imaging, brain computed tomography and Fasekas (F) cognitive test), 16 with A(neurological and cognitive tests: Clinical Dementia Rating (CDR)) and 17 healthy subjects(controls, C). The correlation between parameters was evaluated by Spearman coefficient(r). Results: In L, the advancement of CI correlates with age (r:0.38). CO increased inplasma from L (F2) and A (CDR2), SOD increased in L and A (p<0.01) and CA in L (F3)(p<0.05) without correlation with CI. GSHt in erythrocytes was lower in L and Acompared to C (40% and 92% respectively). In A, IL-6 was higher than in C (p<0.01) andalso than L F2 (p<0.01). In L, CO and IL-6 correlate positively and moderately with thedegree of brain lesions (r:0.27 and 0.55, respectively). In A, CO (r:0.46), total erythrocyteproteins (r:0.35), and TBARS (r:0.40) correlate moderately and negatively with CI. GSHtdecreases with CI in both pathologies (rL: 0.66, rA: 0.87). GSSG in L (r: 0.97), andGSH/GSSG in A (r: 0.89) would be good parameters to evaluate the OS that accompaniesthe progression of the CI. Conclusion: In L, there would be a reversible response tooxidative stress with control of redox homeostasis; while in A, the chronic oxidativeprocesses and OD would be irreversible and with loss of redox homeostasis (oxidativedistress), that worsens with the increase in DI and the progression of the disease.