Nrf2-are signaling pathway associated to antioxidant response in rat brain after acute iron and copper overloads.

REPETTO, M; MUSACCO SEBIO, R; SAPORITO, C; ACOSTA, JM; LAIRION, F; CASTRO PARODI, M; DAMIANO, A; FUDA, J; TORTI, H; FERRAROTTI, N; BOVERIS, A

Paris

Congreso; 9th International Conference on Oxidative Stress Reduction, Redox Homeostasis and Antioxidants; 2017

Introduction: The transcription factor Nrf2-ARE signaling pathway is involved in detoxification and elimination of reactive oxidants through antioxidant and conjugation reactions and by enhancing cellular antioxidant content1. The molecule that seems to be the signal for the genomic transcription is a soluble phospholipid hydroperoxide (ROOH).Materials & Methods: Rats (220 ± 8 g) were given single doses of 30 mg/kg Fe or 5 mg/kg Cu. The activities and expressions of brain cytosolic superoxide dismutase (SOD1), catalase and levels of Nrf2 in brain and the ratio of reduced/oxidized glutathione (GSH/GSSG) was determined in the period of 0-24 h after metals ions loads.Results: SOD1, catalase, glutathione transferase and Nrf2 increased their expressions in response to oxidative stress: SOD1, 2.5 and 1.8 times; catalase 2 and 2.5 times after 24 h (p