Biomarkers of oxidative stress and inflammation associated with differential diagnoses in ageing patients with Leukoaraiosis and Alzheimer disease.

ALEJANDRA CIMATO; MARGARITA MARTÍNEZ SARRASAGUE; FABIANA LAIRION; CHRISTIAN SAPORITO; JORGE SERRA; ENRIQUE MARSCHOFF; LILIANA OUDERK; GRACIELA ADA BIANCHI; RAÚL DOMINGUEZ; MARISA GABRIELA REPETTO

CIUDAD AUTONOMA BUENOS AIRES

Congreso; 66º Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2021

Sociedad Argentina de Investigación Clínica

BIOMARKERS OF OXIDATIVE STRESS AND INFLAMMATION ASSOCIATED WITH DIFFERENTIAL DIAGNOSES IN AGEING PATIENTS WITH LEUKOARAIOSIS AND ALZHEIMER DISEASE.Alejandra Cimato1,2, Margarita Martínez Sarrasague1,2, Fabiana Lairion1,2, Christian Saporito Magriñá1,2, Jorge Serra2, Enrique Marschoff3, Liliana Oudkerk4, Graciela Ada Bianchi4, Raúl Dominguez4, Marisa Gabriela Repetto1,21University of Buenos Aires, Buenos Aires, Argentina, 2Institute of Biochemistry and Molecular Medicine (IBIMOL, UBA-CONICET), Buenos Aires, Argentina, 3Antartic Argentine Institute, Buenos Aires, Argentina, 4University of Buenos Aires, Hospital Sirio-Libanes, Buenos Aires, Argentina.Previous results indicate that oxidative stress (OS) and damage (OD) are associated to the etiopathogenesis of neurodegenerative disorders. Leukoaraiosis (L) is related to the small cerebral vessel disease and mainly associated with aging, dementia, hypertension, cognitive impairment and Alzheimer disease (AD). The aim of this research is to evaluate whether OS and OD are associated with inflammatory response in L and AD patients. Biomarkers of OS, OD and inflammation were evaluated in 33 ageing patients: 17 with diagnosis of L (magnetic resonance imaging and brain computed tomography) and 16 with AD (neurological and cognitive tests), and healthy subjects (controls, n=17). The following parameters were assessed in plasma: protein oxidation measured as carbonyl groups (CO), phospholipid oxidation, measured as the content of thiobarbituric acid reactive substances (TBARS), interleukin 6 (IL-6), total antioxidant (TRAP) and protein content; and in red cells: protein content, superoxide dismutase and catalase activities. A 100% increase of TBARS was observed in L (p