Incubation of frozen‐thawed llama sperm with seminal plasma

FUMUSO, FERNANDA GABRIELA; GIULIANO, SUSANA MARÍA; CHAVES, GRACIELA; NEILD, DEBORAH M.; MIRAGAYA, MARCELO HORACIO; BERTUZZI, MARIANA LUCÍA; CARRETERO, MARIA IGNACIA

ANDROLOGIA

WILEY-BLACKWELL PUBLISHING, INC

Año: 2020 vol. 52

0303-4569

Seminal plasma is intimately connected to sperm physiology and particularly in SouthAmerican Camelids, has demonstrated to be involved in multiple physiological reproductive events. Different percentages of seminal plasma (0%, 10% and 50%) wereadded to thawed llama semen samples with the objective of evaluating the interaction with cryopreserved sperm over time (0, 1.5 and 3 hr at 37°C). A total of 20 ejaculates from five adult llama males (n = 5; r = 4) were evaluated. A significant decrease insperm motility, membrane function and live sperm was observed in all thawed samples (0%, 10% and 50%) at 0 hr when compared to raw semen. Neither morphologynor chromatin condensation was altered in all thawed samples (p > .05), but a significant increase in the percentage of spermatozoa with fragmented DNA was observedafter thawing all samples versus raw semen. When evaluating thawed samples overtime, a significant decrease of motility and membrane function was observed, whilethe percentages of total live sperm were preserved over the 3 hr of incubation in allfinal concentrations evaluated. To conclude, the addition of 10% or 50% of seminalplasma was incapable of preserving motility or membrane function of frozen-thawedllama sperm during 3 hr of incubation