Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa.

CARRETERO MI; NEILD DM; FERRANTE A; CALDEVILLA M; ARRAZTOA CC; FUMUSO FG; GIULIANO SM

ANDROLOGIA

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2015 vol. 47 p. 685 - 693

0303-4569

The aim of this study was to determine the effect of two equilibration temperatures (5º C and room temperature) and two cryoprotectants (glycerol and dimethylformamide, both at 7%) on llama sperm cryopreservation. Llama ejaculates were divided into 4 aliquots. A lactose-EDTA-egg yolk (LEEY) extender with either 7% glycerol (LEEY-G) or 7% dimethylformamide (LEEY-DMF) was added to 2 of the aliquots, which were equilibrated for 20 minutes at room temperature and subsequently frozen. The other 2 aliquots were extended in LEEY, cooled to 5° C, then LEEY-G or LEEY-DMF was added, equilibrated for 20 minutes at 5° C and frozen. No significant differences (P>0.05) were observed in membrane function and chromatin condensation between any of the freeze-thawing protocols. Post-thaw motility was greater (P