Evaluation of the acrosomal status in Lama glama sperm incubated with acrosome reaction inducers

CARRETERO MI; FUMUSO FG; NEILD DM; GIULIANO SM; CETICA P; MIRAGAYA MH

ANIMAL REPRODUCTION SCIENCE

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 160 p. 1 - 11

0378-4320

The objectives of this study were to evaluate the effect of different acrosome reaction (AR) inducers on viability and acrosomal status in llama spermatozoa, by using the FITC-PNA/PI technique and evaluate if there is a positive correlation between the FITC-PNA/PI and the Coomassie blue (CB) staining techniques. After incubating twenty ejaculates in 0.1% collagenase the centrifuged pellets were resuspended in TALP-BSA medium. An aliquot was sonicated to remove the acrosomal content (positive control). The rest of the sample was incubated for 3 h at 38 °C with 5% CO2 and 100% humidity. Treatments: three aliquots were further incubated 1 h with one of the following AR inducers: calcium ionophore, ionomycin or progesterone. Controls: one without inducers and the other, incubated with dimethyl sulfoxide (vehicle of the inducing agents). Acrosomes were evaluated at time 0 and after 4 h incubation. Calcium ionophore was the most potent agent for inducing the AR (67.2 ± 14.4% live + dead AR sperm) (P