EFFECTS OF MINERAL AND VITAMIN SUPPLEMENTATION IN THE OXIDATIVE STRESS BIOMARKER 3-NITROTYROSINE IN PLASMA, URINE AND LIVER TISSUE OF DAIRY CATTLE DURING THE TRANSITION PERIOD

ANGELI, E.; BARCAROLO, D.; REY, F.; ORTEGA, HH.; HEIN, GJ.

Mar del Plata

Congreso; LXVI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2021

SAIC

The intensification of milk production has generated a great metabolicdemand in the animals causing an increase in the oxidative stress, particularlyduring the transition period of dairy cattle. Therefore, we aimed to study theconcentration of free 3-nitrotyrosine (3-NT) in plasma, urine and liver tissue,as a marker of the oxidative stress, and other metabolic biomarkers in plasmaof cows supplemented with vitamins and minerals during the transition period. Thesupplemented group (SG; n = 11) received subcutaneously a dose of 5ml of the vitamin supplement ADAPTADOR® Vit and 5 ml of the mineral supplement ADAPTADOR®Min (Biogenesis Bagó, Bs. As.; vitamin A palmitate 3.5% and vitamin E acetate5%, copper edetate 1%, zinc edetate 4%, manganese edetate 1% and sodiumselenite 0.5%) on -60, -30 and 7 days relative to calving. The control group (CG;n = 11) received two injections of 5 ml of 0.9 % sodium chloride. Blood, urine and liver biopsies were sampled at -21, 7 and 21 days relativeto calving and to evaluate 3-NT.Also, plasma concentrations of non-esterified fatty acids, beta-hydroxybutyrate,glucose, albumin, cholesterol, bilirubin, aspartate aminotransferase, gamma-glutamyltransferase and liver triacylglycerol content were spectrophotometricallymeasured. In cows of the SG, 3-NT concentration was greater in plasma (p < 0.05)and lesser in the liver tissue (p < 0.05). In addition, a lesser livertriacylglycerol content and greater glucose concentration was detected in cowsof the SG (p < 0.05). Regarding albumin, plasma concentration wasgreater in cows of the SG (p < 0.05), with a supplementation xtime effect (p < 0.05), being particularly greater on day 21pre-calving and day 4 post-calving. No statistical differences were evidencedin the other studied parameters. These results suggest that mineral and vitaminsupplementation could ameliorate the oxidative stress in the liver and thetransition of dairy cows.