Alteration of key metabolic components in a follicular persistence model in dairy cows

GAREIS, NC.; CATTANEO, ML.; DÍAZ, PU.; ORTEGA, HH.; SALVETTI, NR.; HEIN, GJ.; REY, F.

Mar del Plata

Congreso; LXVI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2021

SAIC

Several local metabolic factors are involved in the ovarian follicularpersistence associated with anovulation similar to the ovaries from cows withcystic ovarian disease. Therefore, we aimed to studythe protein expression of AdipoR1, AdipoR2, AMPK, CPT1 and ACOX1 in ovarianfollicular structures during the development of follicular persistence in cows.Non-lactating Holsteincows (n=25) with regular estrous cycles without previous reproductive diseasewere studied. The persistence model was performedwith an intravaginal progesterone device to get subluteal concentrations ofprogesterone, obtaining dominant follicles by ovariectomy at the expected dayof ovulation (n=5; P0) and follicles that persist for 5 (n=5; P5), 10 (n=5;P10) or 15 days (n=5; P15) relative to the expected time of ovulation. Controlscows, without progesterone treatment, were ovariectomized in proestrus (n=5;C). Expression of target protein was assessed by indirectimmunohistochemistry in ovarian tissue sections. Intheca cells, AdipoR1 expression was higher in persistent follicles of P15 than persistent/dominantfollicles of other groups (p<0.05). AdipoR2 expression in theca cells was higherin persistent follicles of P15 than follicles of groups C and P0 (p<0.05). AMPKexpression tends to increase (p=0.078) in the granulosa cells of the persistentfollicles of the P15 group with respect to persistent/dominant follicles of theother groups. In granulosa cells, the expression of CPT1 was higher inpersistent follicles of P5, P10 and P15 groups than C and P0 groups(p<0,05). In theca cells, the expression was higher in persistent folliclesof P15 than C, P0 and P5 groups (p<0.05). ACOX1 expression showed nodifferences (p>0.05) in follicular structures analyzed. These resultsevidence a local alteration in some metabolic sensors from the initial stages of persistence, possibly related toalterations in the oxidation of fatty acids at intraovarian level.