Effect of ACTH on the preovulatory period in cattle and its influence on the expression of TGFB and its receptors in the ovary

PEUST CS; BARALE J; BELOTTI EM; ORTEGA HH; SALVETTI NR; HEIN GJ; MATILLER V

Mar del Plata

Congreso; LXIII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

SAIC

The components of thetransforming growth factor-beta (TGFB) superfamily exert functions related tofollicular development, ovulation and corpus luteum formation. We found analtered expression of these components in the ovaries of animals with cystic ovariandisease (COD). In addition, dairy cattle are exposed daily to stressors thatcan affect reproductive function. Therefore, the aim of this study was todetermine the protein expression of the TGFB isoforms (TGFB1, 2, 3) and itsreceptors (TGFBR1, 2, 3) by immunohistochemistry in different follicular categoriesin granulosa and theca interna cells from the ovaries during the preovulatoryperiod of cattle. Animals were treated with adrenocorticotropic hormone (ACTH) every12 hours for five days before the ovulation. We detected a higher expression ofTGFB1 in granulosa cells of large preantral follicles of the ACTH-treated groupcompared to those of the control group. TGFB2 and TGFB3 showed a higherexpression in theca cells of dominant follicles of the ACTH-treated group comparedto antral follicles of control group (p<0.05). TGFBR1 was more expressed inthe granulosa and theca cells of the dominant follicles of the ACTH-treated groupthan in the antral follicles of the control group. Furthermore, its expression washigher in granulosa cells of primary and primordial follicles of the ACTH-treatedgroup than those of the control group. A lower expression of TGFBR3 was detectedin theca interna cells of dominant follicles of the ACTH-treated group comparedto dominant and antral follicles of the control group (p<0.05).These resultscontribute to that previously published for animals with COD. The changesdetected incipiently in animals stimulated with ACTH could contribute to afailure in ovulation and, consequently, with the pathogenesis of diseases occurringwith anovulation as one of its signs.