Alterations of adiponectin receptors and 5-AMP kinase expression in follicular structures of cows with cystic ovarian disease

GAREIS NC; DÍAZ PU; SALVETTI NR; PANZANI CG; ORTEGA HH; HEIN GJ; REY F

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016

SAIC

Adiponectin is an adipokineproduced by adipose tissue which regulates several reproductive processes. Itsrole in steroidogenesis, considering the energy balance for the ovary functions,could be potentially associated to the development of reproductive disorderssuch as cystic ovarian disease (COD). The aim of this study was to determinethe expression of components of the adiponectin system in ovarian follicles ofcows with spontaneous COD and control cows. Thus, the receptors of adiponectin,AdipoR1 and AdipoR2, and the enzyme 5-AMP kinase (AMPK) were evaluated. Animalswith spontaneous COD (n=8) and control cows (n=8) were ovariectomized andovaries obtained were processed histologically to embedded in paraffin.Expression of target protein was assessed by indirect immunohistochemistry onovarian tissue sections. Results showed similar expression of AdipoR1 infollicular structures evaluated in ovaries from control cows and cows with COD,showing a tendency to decrease the expression in granulosa cells of cysts regarding to antral follicles (as referencestructure) of control cows (p<0.1). However, a higherexpression of AdipoR2 was detected in theca cells of cystscompared to antral follicles of control cows (p<0.05) without differences ingranulosa cells. Moreover, the expression of AMPK in theca cells of cysts washigher compared to control antral follicles (p<0.05) without differences ingranulosa cells of the follicles analyzed. The results of the present studyshowed variations in proteins involved in response and signaling to adiponectin.Considering the association of this adipokine with several metabolic functions,such as improving the insulin sensitivity, and also its essential role for folliculargrowth and maturation, the alterations here detected could indicate a potentialcontribution of this cytokine in ovarian function related to reproductivedisorders.