INVESTIGADORES
HEIN Gustavo Juan
congresos y reuniones científicas
Título:
KEY ENZYMES AND NUCLEAR RECEPTORS OF HEPATIC LIPID METABOLISM IN DAIRY CATTLE DURING THE TRANSITION PERIOD
Autor/es:
ANGELI E; TRIONFINI V; GAREIS NC; RODRIGUEZ FM; MARELLI BE; HEIN GJ; ORTEGA HH
Lugar:
Mar del Plata
Reunión:
Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016
Institución organizadora:
SAIC
Resumen:
Thetransition period (TP) is the most critical in the lactation of a dairy cow,characterized by a negative energy balance leading to an important lipidmobilization, with increases in the systemic non-esterified fatty acids (NEFA)and beta-hydroxybutyric (BHB) concentrations. While carnitinepalmitoyltransferase-1 (CPT1), acyl-Coa oxidase-1 (ACOX1) and the nuclearreceptor PPAR-alpha promotes fatty acids oxidation in several tissues,including the liver, diacylglycerol acyltransferase-1 (DGAT1) is involved intriacylglycerol biosynthesis. The aim of this study was to evaluate somepathways of hepatic lipid metabolism during the TP. Blood samples and liverbiopsies from cows (n = 10) were taken at 28 and 14 days prepartum, and also at4, 14, 28 and 60 days postpartum. Systemic NEFA, BHB, glucose and insulin concentrationswere measured. Besides, the liver expressions of fatty acids oxidation (CPT1,ACOX1) and re-esterification (DGAT1) enzymes, and nuclear receptor PPAR-alphawere evaluated by Real Time-PCR and western blot. There were no significantchanges (p > 0.05) in glycemia and insulinemia. Differences in the BHB andNEFA concentrations were observed at different sampling days, being significantat 4, 14 and 30 days postpartum (p < 0.05). The expressions of CPT1 and PPAR-alphawere higher in the postpartum period (p < 0.05), with an opposite behaviorrespect the expression of DGAT1. No differences in the mRNA expression of ACOX1were observed (p > 0.05). These results show significant changes in hepaticlipid metabolism of dairy cows during the TP. These knowledge could represent auseful tool to understand the metabolic behavior in animals with different milkproduction and to optimize the health of dairy cattle during this period.
