KEY ENZYMES OF LIPID AND CARBOHYDRATE METABOLISM IN LIVER OF DYSLIPEMIC RATS: EFFECT OF FISH OIL

HEIN, G.J.; CHICCO, A.G.; LOMBARDO, Y.B.

ROSARIO - ARGENTINA

Congreso; XLII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN EN BIOQUÍMICA Y BIOLOGÍA MOLECULAR; 2006

SOCIEDAD ARGENTINA DE INVESTIGACIÓN

Objectives: The present study analyses different enzymes involved in the synthesis and oxidation of fatty acid in the liver and its relationship with glucose metabolism. Male Wistar rats were fed a SRD (w/w: 63% sucrose, 8% corn oil (CO), 17% protein) for 6 months. After that, one half of the rats continued with the SRD up to 8 months, while the other half received a SRD in which the CO was substituted by cod liver oil (CLO) (w/w: 7% CLO plus 1% CO) from months 6 to 8. Control rats received a control diet (CD) (w/w: 63corn-stach, 8% CO, 17% protein) for 8 months. We studied a) plasma: glucose, insulin, Tg, AGNE, b) liver: Tg and glycogen content and enzymatic activities of ME, FAS, FAO, GSa, GK, G-6-PPase. Results: SRD vs CD: Increased plasma Tg, AGNE and glucose levels p<0.05. Liver: Increased: 1) Tg and glycogen content, 2) ME, FAS and GSa activities and the rate of G-6-PPase/GK. The addition of CLO normalized plasma Tg, AGNE and glucose levels. The liver Tg content as well as ME, FAS and GSa activities reached normal values, while FAO activities increased. Conclusions: The altered pattern of lipid and carbohydrate metabolism of SRD fed rats tended to be normal with the administration of cod liver oil.