Oxidative stress biomarkers and pathways of hepatic lipid metabolism in dairy cattle supplemented with minerals and vitamins during the transition period

ANGELI E; BARCAROLO D; CANAVECIO J; REY F; MATILLER V; ORTEGA HH; HEIN GJ

Mar del Plata

Congreso; Reunión Conjunta SAIC - SAI - SAFIS - Noviembre 2022; 2022

SAIC

The transition period is the most critical stage inthe lactation of a dairy cow, characterized by lipid mobilization and a greatmetabolic demand that could increase oxidative stress (OS). Therefore, thisstudy aimed to evaluate some OS parameters and pathways of hepatic lipidmetabolism in cows supplemented with vitamins and minerals during this period. Thesupplemented group (SG; n = 11) received a subcutaneous dose of 5 mlof the vitamin supplement ADAPTADOR® Vit and 5 ml of the mineral supplement ADAPTADOR®Min (Biogenesis Bagó, Bs. As.; vitamin A palmitate 3.5% and vitamin E acetate5%, copper edetate 1%, zinc edetate 4%, manganese edetate 1% and sodiumselenite 0.5%) on -60, -30 and 7 days relative to calving. The control group (CG;n = 11) received two injections of 5 ml of 0.9% sodium chloridesolution. Blood,urine and liver biopsies were sampled at -21, 7 and 21 days relative to calving,and malondialdehyde (MDA) was evaluated by mass spectrometry as an OS biomarker.In addition, serum concentrations of non-esterified fatty acids (NEFA), beta-hydroxybutyrate acid (BHBA) and the total antioxidantstatus (TAS) were determined by using commercial colorimetric assay kits. Finally,triacylglycerol content was measured spectrophotometrically, and proteinexpressions of carnitine palmitoyltransferase-1 (CPT1), acyl-CoA oxidase-1(ACOX1); diacylglycerol acyltransferase-1 (DGAT1) and nuclear receptorPPAR-alpha were evaluated in liver biopsies by Western blot. No differencesin NEFA and BHBA concentrations were observed (p > 0.05). However,a lesser hepatic triacylglycerol content was registered in cows of the SG (p < 0.05).Regarding liver enzymes, a greater protein expression of CPT-1 was observed in theSG (p < 0.05) without differences in the other enzymes evaluated(p > 0.05). Similar MDA concentrations and TAS were observed(p > 0.05). These results suggest that mineral and vitaminsupplementation could influence liver physiology by improving the adaptation ofthe dairy cow during the transition.