BMP2, 4, 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development

DÍAZ PU (EQUAL CONTRIBUTION); HEIN GJ (EQUAL CONTRIBUTION); BELOTTI EM; RODRIGUEZ FM; REY F; AMWEG AN; MATILLER V; BARAVALLE ME; ORTEGA HH; SALVETTI NR

REPRODUCTION

BIOSCIENTIFICA LTD

Lugar: Bristol; Año: 2016 vol. 152 p. 333 - 350

1470-1626

Cystic ovarian disease (COD) is an important cause ofsubfertility in dairy cattle. Bone Morphogenetic Proteins (BMPs), mainly BMP2,BMP4, and BMP6, play a key role in female fertility. In this study, we hypothesizedthat an altered BMP system is associated with ovarian alterations contributingto COD pathogenesis. Therefore, we examined the expression of BMP2, BMP4, BMP6and BMP receptor 1B (BMPR1B) in the ovaries of animals with spontaneous orACTH-induced COD, as well as during the development of the disease, in a modelof follicular persistence induced by low doses of progesterone (at 5, 10 and 15days of follicular persistence). Results showed changes in BMP2, BMP4 and BMP6 expressionduring folliculogenesis, in granulosa and theca cells in the COD groups, aswell as at the different stages of follicular persistence. Results also showedchanges in BMPR1B expression in developing follicles in animals with COD, and atthe initial stages of follicular persistence (P5). Comparison between groupsshowed significant differences, mainly in BMP4 and BMP6 expression, ingranulosa and theca cells of different follicular categories. The expression ofthese BMPs was also increased in cystic and persistent follicles, in relationto antral follicles of the control group. BMPR1B showed high expression incystic follicles. Together, these results may indicate an alteration in BMPs,especially in BMP4 and BMP6, as well as in BMPR1B, which occurs early infolliculogenesis and incipiently during the development of COD, and which couldbe a major cause of recurrence of this disease in cattle.