CONICET | Buscador de Institutos y Recursos Humanos

Fish processing generates plenty of waste that is directly discarded on open-air dumpsand water sources, or treated in the same way as urban solid waste causing seriouspollution problems. The waste represents a significant source of high-valuebioproducts with potential applications in different industrial processes such as theproduction of feed, fertilizers, biodiesel and biogas, detergent additives and cosmetics.The objective of this study was to characterize and to compare specific activities underdifferent pH values and temperature conditions of acid and alkaline proteinases andviscera yield from the following fish species: Argentine hake Merluccius hubbsi ,Brazilian flathead Percophis brasiliensis , Brazilian codling Urophycis brasiliensis ,and Stripped weakfish Cynoscion guatucupa . Individuals were fished off the coast ofMar del Plata (Argentina) by a commercial fleet and the viscera were immediatelyextracted and kept on ice until use. Stomach proteinases from four species had thehighest activity at pH 2, with stability in the range of pH 2-4. The optimum pH was 11.5from intestinal enzymes of C. guatucupa, M. hubbsi and P. brasiliensis and 9.5 fromintestinal enzymes of U. brasiliensis . Alkaline proteinases from all species werehighly stable in the range of 7-11.5. The optimum temperature of stomach proteinasesfrom the four species studied were 30 °C and 50 °C, with stability at 10 °C and 30 °Cduring 150 min. The optimum temperature of intestinal enzymes from the testedspecies were 50 °C with high stability at 10 °C and 30 °C during 150 min. Alkalineproteinase from all species and acid proteinases from C. guatucupa were inactive at70 ºC after 150 min; while there was a residual activity lower than 5 % at 80 °C on preincubated stomach enzymes of M.hubbsi , P. brasiliensis and U. brasiliensis after5, 10 and 20 minutes, respectively. Digestive proteinases recovered in this study couldbe appropriate for technological usage, reducing manufacturing costs, obtainingrevenue from fishery wastes, and contributing to the reduction of environmentalpollution.

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