The effect of dietary vitamin D3 on the midgut gland histomorphology of the cultured shrimp Pleoticus muelleri.

PEREIRA N.D.A; FERNÁNDEZ GIMENEZ, ANALIA VERÓNICA; FENUCCI, J.L

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2013 vol. 37 p. 34 - 34

0327-9545

Food is the most influential factor in the physiological condition of crustaceans. Liposoluble vitamins are essential to keep the nutri- tional and sanitary status in perfect conditions; vitamin D3 is very important for the calcium metabolism. The midgut gland (HP) pre- sents 4 cell types: B, E, F, and R. The last two react with different diets, being indicators of nutritional stress. This research has evalu- ated the effect of different levels of vitamin D3 in the HP of the shrimp Pleoticus muelleri through 5 semipurified diets: 0, (D0); 8.000 (D8); 15.000 (D15); 30.000 (D30) and 40.000 (D40) UI vi- tamin D3/kg diet. P14 was used as a factor of control, nutritionally complete diet. Groups of 8 individuals of 4 grs were tested during 9 weeks within aquariums of 150 liters (salinity 33%; 18ºC; pH 7; 11:13 hs l-d). At the end of each experiment, the animals were ani- mals were crioanesthesiated and were determined of molting stage. The HP of shrimp in intermolt stage were extracted, fixed and pro- cessed for light microscopy. The result of the histological study showed that the HP of the shrimps fed on diets D0 and PL4 pre- sented well- preserved morphology and cytology. In the rest of the diets, a rise in the cellular and histomorphological deterioration was observed in the following order: D8< D30