Participation of sex chromosome complement in brain pattern activation during acute sodium depletion

DADAM FLORENCIA; CAEIRO XIMENA; CISTERNAS CARLA; VIVAS LAURA; CAMBIASSO MARÍA JULIA

Córdoba

Congreso; XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; 2011

Sociedad Argentina de Investigación en Neurociencia

In order to evaluate whether sex chromosome complement (SCC) differentially modulates brain activity during acute sodium depletion (DEP) we used the 4 core genotype mouse model in which the effect of gonadal sex and SCC is dissociated, allowing comparisons among XX and XY females as well as in XX and XY males. Gonadectomized male and female mice were submitted to DEP by a combined treatment of the diuretic furosemide and a low sodium diet. Twenty one hours later, the mice were perfused and their brains were subjected to Fos inmunoreactivity (Fos-Ir) procedure. The statistical analysis shows that DEP elicits a significant increase in the Fos-Ir at the vascular organ of the lamina terminalis and median preoptic nucleus irrespectively of sex and CCS factors. Activation of subfornical nucleus however is affected by the interaction of treatment and CCS. At brainstem level, the nucleus of the solitary tract and parabrachial lateral nucleus shows a similar pattern of activation since mice bearing SCC-XX show an increased neuronal activity at both nuclei. Furthermore, DEP-XX males show a higher Fos-Ir at the area postrema level. Our results indicate that SCC may modulate brain activity of nuclei closely involved in hydrosaline homeostasis. Funding: ANPCyT, CONICET, Roemmers and FUCIBICO.