EFFECT OF EARLY MATERNAL SEPARATION ON BLOOD PRESSURE REGULATION IN MALE AND FEMALE MICE

SILVA GABRIELA VANINA; GODINO ANDREA; RIVAROLA MARÍA ANGÉLICA; CAEIRO XIMENA

Buenos Aires

Congreso; Reunión Anual 2023-SAFIS; 2023

SAFIS

EFFECT OF EARLY MATERNAL SEPARATION ON BLOOD PRESSURE REGULATION IN MALE AND FEMALE MICESilva GV1, Godino A1, Rivarola MA2, Caeiro XE11Instituto de Investigación Médica Mercedes y Martín Ferreyra (INIMEC-CONICET-Universidad Nacional de Córdoba)2 Instituto de Investigaciones en Ciencias de la Salud (INICSA, CONICET, Universidad Nacional de Córdoba)Introduction Evidence in the experimental and clinical field have shown that during sensitive periods of ontogeny, certain perinatal stimuli may induce “differential programming effect” on homeostatic systems, altering their response even during adulthood.ObjectivesWe sought to analyze the influence of sex and early maternal separation (EMS) programming on mean arterial pressure (MAP) regulation in response to intravenous vasopressin and hypertonic NaCl continuous infusion in adult MF1 male (M), female in diestrus and in female in proestrus.MethodsIn the EMS group, litters were daily separated from their dams for 3 hours each day between postnatal day 2 until day 14, while in the control groups (CON) the offspring remained with their dams. In adult male, female in diestrus and in proestrus mice aged 73 to 83 days old (from EMS and CON groups), changes in blood pressure induced by intravenous 30 min-continuous infusion of vasopressin (1; 00μl 0.01 IU/100ul) and hypertonic NaCl solution (100μl, 3.4 M NaCl) were analyzed. ResultsThe statistical analysis showed that, although vasopressin continuous infusion induced the expected increase in MAP in all groups, no differences among EMS and CON groups were observed in males, however significant differences attributable to the interaction of estrous cycle (proestrus and diestrus), treatment and time were observed in females {F(8,192)= 2.535, p=0.02}; with females in diestrus-CON group presenting a smaller increase in MAP when compared to female in diestrus-EMS and female in proestrus-CON groups.Furthermore, the continuous infusion of hypertonic NaCl in female in proestrus resulted in a significant effect of the interaction of the time and treatment factors {F(12,103)=3.1444, p=0.00073}, with a decrease in MAP in the EMS group from 15 minutes after ending NaCl hypertonic infusion when compared to the CON group. However, in male and female in diestrus, no differences among EMS and CON groups were observed.ConclusionThese results demonstrate sex differences in blood pressure regulation in CON and EMS groups, with an influence of the estrous cycle in females on MAP response to both AVP infusion and osmotic challenge.Area temática: FISIOLOGÍA CARDIOVASCULAR-HIPERTENSIÓN