Diagnosis of Trichinellosis by ELISA test with three types of antigens of Trichinella spiralis in pigs raised under different conditions of confinement.

RIBICICH,M; GAMBLE, H.R.; BOLPE, J.; CARDILLO, N.; PASQUALETTI, M.; SAN MARTÍN, C.; VIZIO, E.; BORRÁS, P.; FARIÑA, F.; MUNDO, S.; ROSA, A.

The Pig Journal

The Pig Veterinary Society

Lugar: West House Station Road Thirsk; Año: 2011 vol. 66 p. 55 - 58

1352 9749

In Argentina, many pigs are raised outdoors and Trichinella infection is endemic. In this report, 21 pig farms were selected for study including some using total and partial confinement management, some where pigs were raised in outdoor housing and some where pigs were raised under poor hygienic and sanitary conditions. A total of 3224 muscle samples were collected from pigs on the 21 farms and these muscle samples were used to determine the presence of T. spiralis larvae by artificial digestion (DAR). Serum samples from these same 3224 pigs were tested for antibodies to T. spiralis using three ELISA tests. The laboratory ELISA, the Microwell ELISA and the Glycan Microwell ELISA had sensitivities and specificities of 100% and 100%, 95.5% and 100%, and 95.5% and 100% respectively when compared with results of artificial digestion testing. Assuring that pigs are free from Trichinella infection is a priority for the pork processing industry in Argentina. Permanent monitoring systems performed in pig establishments, including the use of serology tests, as we describe here, would help to reduce or eliminate the risk for human exposure to Trichinella in pork.