Combined trapping: tobramycin + Thymus vulgaris essential oil within nebulizable ultra small nanostructured lipid nanoparticles

PEREZ, N; ALTUBE, MJ; PEREZ, AP; ROMERO, EL

Mar del Plata

Congreso; Reunión anual de sociedades de biociencia SAIC-SAFE-SAB-SAP- AACYTAL-Nanomed-ar-HCS 2019; 2019

SAIC-SAFE-SAB-SAP- AACYTAL-Nanomed-ar-HCS

Essential oils (eo) are mixtures of volatileterpenes having pleiotropic activity on multiple therapeutictargets. Their use as antibiotics or antitumorals however, islimited by a high physicochemical unstability and toxicity foreukaryotic tissues. Aiming to solve such challenges, in this workwe explore the concept of combined trapping of actives withintargeted nanoparticles. Specifically, there were preparednanostructured lipid nanoparticles (NLN) covered with lipidsurfactants (total polar archaeolipids, TPA) extracted from thehyperhalophilic archaebacteria Halorubrum tebenquichense(NLN-TPA). The nanostructure of such nanoparticles resultedefficient to entrap and protect variable amounts of Thymusvulgaris eo (eoT) together with the hydrophilic antibiotictobramycin (TB) and were named as ?NLN-TPA (eoT+TB)?. Ourpreliminary data showed that NLN-TPA (eoT+TB) made of [(2 %)compritol: (0.5 %) miglyol: (1 %) TPA: (3 %) Tween 80: (89 %)H2O: (5 %) eoT: (0.03 %) TB] were structurally stable along 6 ofstorage (~ 300 nm Z average, 0,45 pdi, ~ -40 mV) andimportantly, to the nebulization stress. Remarkably, the presenceof TPA was critical to stabilize the combined trapping of the twoactives. Besides, the presence of TPA provides a selective targeting to cells expressing SR-AI/II, such as macrophages anddendritic cells, as well as optimizes the interaction with bacterialbiofilms in the respiratory tree. It is expected that in NLN-TPA,the combined trapping of eoT and TB would magnify theantibacterial activity of both agents, providing higher therapeuticactivity that the achieved by TB alone and at doses small enoughof eoT to be innocuous for eukaryotic cells.