Characterization and interaction with cells of nanohybrids: ZnO nanorods - ultradeformable nanovesicles

PEREZ AP; APEZTEGUIA G; ROMERO EL

Congreso; Reunión conjunta 2016 SAIC-SAI-SAFE-AACyTAL-NANOMEDar; 2016

SAIC-SAI-SAFE-AACyTAL-NANOMEDar

The efficient penetration of adjuvant and antigen across theskin can generate antigen-specific protective immune reactionsrequired for development of a topical needle free vaccine. Theobjective of this work was to design an adjuvant system combiningthe ability of ZnO nanorods (NRZnO) to generate reactive oxygenspecies (ROS), and the ability of ultradeformable nanovesicles(ultradeformable liposomes and archaeosomes- UDL and UDA,respectively) to penetrate skin. This combination would facilitatethe access of NRZnO to the viable epidermis triggering an immuneresponse against an antigen associated. In this work, NRZnOsuspensions and nanohybrids (NRZnO-UDL and NRZnO-UDA)were prepared and characterized regarding colloidal stabilityandinteraction with cells in vitro. The aqueous suspension ofNRZnO presented reproducible structural characteristics in termsof zeta potential, absorption of UV light, hydrodynamic size andpolydispersity, remained stable against changes in ionic strengthand pH neutral to basic and generated ROS. However, large aggregateswere formed after storage at 4°C for 7 to 28 days. Incontrast, not formation of aggregates was found after storage ofnanohybrids, indicating that the combination with nanovesiclesprevents aggregation of NRZnO. On the other hand, cytotoxicity,uptake and proinflammatory cytokine production were performedin keratinocytes HaCaT and macrophages J774A.1 and THP-1.Only macrophages were able to uptake NRZnO. Finally, incubationof macrophages with NRZnO induced the production of proinflammatorycytokines IL-1β in THP-1 and TNF-α in THP-1 andJ774. However, cytokines were not released or potentiated afterincubation with nanohybrids, probably due to a high quantity ofnanovesicles compared to the quantity of NRZnO used, whichcould decrease uptake of NRZnO. In conclusion, nanohybrids were effective to achieve colloidal stability but cytokines releasewas not potentiated compared to NRZnO.