CHARACTERIZATION AND ANTIBIOFILM ACTIVITY OF LIPID NANOVESICLES CONTAINING ESSENTIAL OIL OF THYMUS VULGARIS

PEREZ ANA PAULA; PEREZ NOELIA; SULIGOY MAURICIO; ALTUBE MARIA JULIA; MARCELO ALEXANDRE DE FARIAS; RODRIGO VILLARES PORTUGAL; BUZZOLA FERNANDA; ROMERO EDER LILIA

Mar del Plata

Conferencia; SAI-SAIC-SAFIS 2018; 2018

The clinical relevance of bioflms (matrix-enclosed communities ofbacteria that are adherent to surfaces) is related to their wide occurrence together with their ability to cause relapsing infections characterized by chronic inflammation, tissue damage and the signifcantdiffculties in their eradication. The antibiotic tolerance shown bybacteria in the bioflm has been attributed mainly to the matrix, thatrestricts the access of antibiotics by binding them to their components or inactivating them by enzymes. In that way, lipid nanovesicles containing antimicrobial agents (AMA) could penetrate thebioflm matrix and remain there releasing AMA in high doses.We have recently shown that the presence of total polar archaeolipids of Halorubrum tebequichense (TPA) in lipid nanovesiclesincreased their chemical and colloidal stability. We hypothesizedthat the encapsulation of essential oil of Thymus vulgaris (AETv - apotent AMA that has low solubility in water and rapid decomposition)in archaeolipid nanovesicles would allow to obtain a stable aqueous suspension of AETv with effcient antibacterial and antibioflmactivity.Archaeolipid nanovesicles (soy phosphatidylcholine: TPA: Tween 801:1:1.6 w:w) of 129 ± 23 nm, Z potential -7 ± 1.5 mV and nanospherical structure, containing 41.6 ± 14.6 mg/ml of AETv (Arc-AETv) wereobtained by the lipid flm hydration method. The encapsulation ofAETv in Arc-AETv increased the solubility of AETv 20.8 times withregards to an aqueous suspension. After 150 days of storage, ArcAETv maintained the concentration of AETv and was stable colloidally. Finally, Arc-AETv presented antibacterial activity on bothplanktonic S. aureus and bioflm at lower concentrations than Tween80 1% v/v suspension of AETv and AETv in lipid nanovesicles lacking TPA (Lipo-AETv).In conclusion, the incorporation of AETv in archeolipid nanovesiclesfavored obtaining a stable formulation with effective antibioflm activity.