The interaction of nano-archeasomes with lung surfactant in monolayer biomimetic systems

ALTUBE MARÍA JULIA; CUTRO ANDREA; DISALVO ANIBAL; ROMERO EDER LILIA

Tucuman

Congreso; XLV Reunion Anual SAB; 2016

Sociedad Argentina de Biofisica

The archaeosomes (ARC) are lipid vesicles made of total polar archaeolipids(TPA) extracted from hyperhalopile archaebacteria (1). Previousstudies have shown that ARC are more extensively captured by alveolarmacrophages and lung epithelial cells in vitro than conventional liposomes(2). This makes ARC excellent candidates for delivering drugsto the lungs. Although, in an in vivo context inhaled ARC must firstinteract with the pulmonary surfactant (PS) lining layer that covers theinternal surface of the alveolus and provides the low surface tension atthe air-liquid interface that is necessary to prevent collapse during expiration.Interactions with the PS film determine the subsequent retentionor translocation of the inhaled ARC and hence their potential activityon target cells (3). In order to evaluate the effect of the interaction ofARC, the changes in surface pressure (π) and compressibility producedin lung surfactant monolayers by ARC addition were studied. The resultsshow that ARC increases the π, indicating its incorporation whichis dependent of the membrane packing because the ∆π observed at highsurface pressure (40 mN/m) are negligible in comparison to those obtainedat low surface pressure (10 mN/m), (∆π=4.5 mN/m). Besides,the ARC concentration is crucial to produce changes in pressure monolayers,at low concentration (0.3 µg/ mL) no significant changes wereobserved. Moreover, the ARC incorporation rates into the membranewas substantially affected if the addition done onto the monolayer incomparison to that in the subphase being the first much higher than thesecond.References(1)Gonzalez, R.O., Higa,L. H., Cutrullis, R.A., Bilen, M., Morelli, I., Roncaglia, D.I., andRomero, E.L. (2009). BMC biotechnology, 9(1), 71.(2) Altube, M.J., Selzer, S.M., de Farias, M.A., Portugal, R.V., Morilla, M.J., Romero, E.L.(2016). Nanomedicine,11(16):2103-17.(3) Gehr, P., and Tsuda, A. (2014). Nanoparticles in the Lung: Environmental Exposure andDrug Delivery, 21.