Surviving nebulization-induced stress: dexamethasone in pH-sensitive archaeosomes

ALTUBE MARÍA JULIA; SELZER SOLANGE MAILEN ; ALEXANDRE DE FARIAS MARCELO ; VILLARES PORTUGAL RODRIGO ; MORILLA MARÍA JOSE; ROMERO EDER LILIA

NANOMEDICINE

FUTURE MEDICINE LTD

Lugar: Londres; Año: 2016

1743-5889

Aims. To increase the subcellular delivery of dexamethasone phosphate (DP) and stability to nebulization stress, pH-sensitive nanoliposomes exhibiting archaeolipids acting as ligands for scavenger receptors (pH-sensitive archaeosomes (ApH) were prepared. Materials & methods. The anti-inflammatory effect of 0.18 mg DP/mg total lipid, 100-150 nm DP containing ApH (dioleylphosphatidylethanolamine (DOPE): Halorubrum tebenquichense total polar archaeolipids (TPA): cholesterylhemisuccinate (CHEMS) 4.2:2.8:3 w:w) was tested on different cell lines. Size and HPTS retention of ApH and conventional pH-sensitive nanoliposomes (LpH, DOPE:CHEMS 7:3 w:w) before and after nebulization were determined Results & conclusions. DP-ApH suppressed IL-6 and TNF-α on phagocytic cells. Nebulized after 6-month storage, LpH increased size and completely lost its HPTS while ApH3 conserved size and polydispersity, fully retaining its original HPTS content