Characterization of Salmonella enterica serovars Typhimurium and 4,5,[12]:i:- isolated from pig and pig enviromental-related sources in NE of Spain

SARA ANDRES BARRANCO; VICO, J.P; HERRERO-LEÓN S.; RAUL C. MAINAR-JAIME

Oporto

Congreso; SafePork2015; 2015

SafePork

A total of 117 SAlmoenlla Typhimurium and 59 monophasic Salmonella Typhimurium (S. 4,[5],12:i:- strains isolated from pig, wild bird, rodent, and farm enviromental samples were characterized by phage typing, antibiotic susceptibility testing and Multiple-Locus Variable.Number Tandem Repeat Analysis (MLVA) in order to evaluate their phenotypic and genetic relatedness. In S. 4,[5],12:i:- the most prevalent phage types werte U311 (40.7%) and DT195 (22%) which did not correspond with so-called Spanish-clone, and generally showed a different resistant pattern (ASSuT). Antibiotic resistance was found in 85.8% of the isolets, with 94.1% of them displaying multidrug resistance (MDR). MLVA indentified 92 different profiles, six of them shared by both serovars. The Minimum Spanning Tree showed one major cluster that included 95% of the S. 4,[5],12:i:- isolates, which came from different animal sources, geographic locations and time periods, suggesting high clonality among those Salmonella starins and the ability to spread among pig farms. Phenotypically, isolates of S. 4,[5],12:i:- were closer to European strains than to well-characterized Spanish clone. The spread of these new strains of S. 4,[5],12:i:- would have been likely favoured by the important pig trade between this Spanish region and other European countries.