Effect of combined treatment of Interferon αlfa-2b (IFN) and Vitamin E (E) or Quercetin (Q) on the development of liver cancer

LUCCI, A; COMANZO, CG; VERA, MC; LORENZETTI, MF; CEBALLOS, MP; QUIROGA, AD; ALVAREZ, ML; CARRILLO, MC

Congreso; Sociedad Argentina de Investigaciones Clinicas; 2018

Our group has postulated IFN as an effective antitumor agent inthe treatment of carcinoma hepatocellular (HCC). Besides, E andQ were shown to have inhibitory effects on liver cancer due to itsantiangiogenic and antiproliferative activities. We aimed to evaluatewhether the combined therapy of IFN with E or Q has a synergisticinhibitory effect on liver cancer development compared with eachseparate therapy. We treated human liver cancer cells SK-Hep 1with 10000 U/I of IFN, 25 uM of δ-Tocotrienol, an isomer of E, and12,5 uM of Q. Treatments were used alone (IFN-group, E-group andQ-group) or combined (IFN-E-group and IFN-Q-group). Durationof the treatments varied according to the experiment that was carriedout. We perform the MTT assay to determine cell viability at 72hours, the wound healing assay to determine migration at 16 hoursand invasion, in transwell chambers, at 24 hours. As expected,IFN-E and IFN-Q-groups showed a higher decrease in cell viability(-35 %*& and -52 %*# , respectively) compared to monodrug therapy:IFN-group (-25 %*), E-group (-30 %*) and Q-group (-15 %*). Inmigration assay IFN-E-group did not show a significant decrease(-35 %*) compared to monodrug therapy: IFN-group (-28 %*) andE-group (-30 %*), but IFN-Q-group showed a significant decrement(-46 %*&) compared with individual therapy: IFN-group (-28 %*) andQ-group (- 25 %*). In Invasion assay IFN-E-group and IFN-Q-groupshowed a significant diminution (-93 %*#) and (70 %*&) respectively.The monodrug groups showed lower decreases: IFN-group (-25%*), E-group (-14 %*) and Q-group (- 7 %*), (*p