INVESTIGADORES
QUIROGA Ariel Dario
artículos
Título:
Tumor necrosis factor alpha induced by Trypanosoma cruzi infection mediates inflammation and cell death in the liver of infected mice
Autor/es:
RONCO, MARIA TERESA; FRANCES, DANIEL ELEAZAR ANTONIO; INGARAMO PAOLA I; QUIROGA, ARIEL DARIO; ALVAREZ, MARIA DE LUJAN; PISANI, GERARDO BRUNO; REVELLI, SILVIA; CARNOVALE, CRISTINA ESTER
Revista:
CYTOKINE.
Editorial:
ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD
Referencias:
Año: 2010 p. 64 - 72
ISSN:
1043-4666
Resumen:
Trypanosoma cruzi (T. cruzi) infected C57BL/6 mice developed
a progressive fatal disease due to an imbalance in the profile of circulating
related compounds accompanying infection like tumor necrosis factor alpha (TNFa).
TNFa has been proposed as an important effector molecule in apoptosis. In this
work, we evaluate inflammation and the proteins involved in apoptotic process
in liver of infected mice and the role of TNFa. C57BL6/mice were infected
subcutaneously with 100 viable trypomastigotes of Tulahuén strain of T cruzi.
One set of these animals were treated with 375 ug of antihuman TNFa blocking antibody.
Animals were sacrificed at 14 days post-infection (p.i).The analyses of Bcl-2
family proteins revealed an increase of the pro-apoptotic proteins Bax and tBid
in T. cruzi-infected mice. Compared with control animals, cytochrome c release
was increased. Apoptosis was also induced in infected mice. Anti-TNFa treatment
decreases hepatic apoptosis. Our results suggest that T. cruzi infection
induces programmed cell death in the host liver by increase of TNFa production,
associated with TNF-R1 overexpression, that set in motion the Bid cleavage and
mitochondrial translocation, Bax mitochondrial translocation, cytochrome c release,
and ultimately apoptosis induction.