Synthesis of galactofuranosides constituents of Leishmania LPG for antigen-based diagnostic , Junio 2016, Villa General Belgrano, Córdoba.

CAROLINA TOULOUMDJIAN; LUCÍA, GANDOLFI DONADÍO

Villa General Belgrano

Simposio; 2nd Argentinian Symposium of Glycobiology; 2016

Comite Organizador GLYCOAR

Leishmaniasis is a parasitic disease endemic in 98 countries. Diagnostic test that differentiate acutely diseased from clinically asymptomatic patients are highly desired. Developing antibodies to detect directly parasitic cell surface glycans is a promising strategy for diagnostic. The first step of this approach is the elucidation of a right antigenic epitope and produce synthetic well defined structures.1 Lipophosphoglycan (LPG) and glycoinositolphospholipids (GIPLs) represents one of the most abundant surface glycoconjugates, essential for virulence and survival of promastigotes in macrophages and are implicated in immune cell stimulation.2 An unusual feature of LPG and GIPLs is the galactofuranose (Galf) exclusively found in nonmammalian species. Although the important role of this sugar in host-parasite interaction has been studied, no systematic evaluation of immune responses against substructures containing Galf has been conducted.3 Here, we report the synthesis of aminopentyl β-D-galactofuranoside (1) and aminopentyl-β-D-Galf-(1-3)-D-Manp (2), constituents of LPG core and GIPLs. The distinguishing feature of the synthesis is the introduction of the aminopentyl linker at the reducing end to immobilize the sugar in a microarray and investigate the immunological potential by screening against infected sera. Glycoconjugates of the identified epitopes could be test to generate anti-glycan antibodies to be use as the basis of a diagnostic test or potential vaccine.