Synthesis and characterization of solid lipid nanoparticles for controlled release of Carbamazepine

SEBASTIÁN SCIOLI MONTOTO; GERMAN ISLAN; MARÍA ESPERANZA RUIZ; GUILLERMO R. CASTRO

Trivandrum

Conferencia; New Horizons in Biotechnology (NHBT-2015); 2015

CSIR-National Institute for Interdisciplinary Science and Technology (Trivandrum, India) & Biotech Research Society of India (BRSI)

Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) have been shown to penetrate the Blood Brain Barrier and allow for greater delivery of drugs to the CNS. They present the same advantages of polymeric nanoparticles (NP) but with much lower intrinsic toxicity. The aim of the present work was the encapsulation of the antiepileptic drug Carbamazepine in SLN and NLC in order to develop a controlled release delivery system. As lipid phase, cetyl esters and Croda oil® were used. NP were prepared by ultrasonication of a mixture of the melted lipids and 3% solution of P188 in water, previously heated at a temperature slightly above the lipids? mp. The drug was incorporated to the lipid phase, alone or dissolved in 50 mcl of DMSO. Formulations were observed by TEM and AFM, showing a mean size range of 50-500 nm. In vitro release studies were performed in 500 ml of KH2PO4 (pH 6.8) using Apparatus 2 USP (paddle) at 100 rpm. Four different formulations were assayed, and all of them showed high %EE (82.78-93.84%) with released percentages of (29.47-45.30%) and (50.80-78.53%) at 2 and 6 hours, respectively, reaching (70.34-99.59%) at 24 hours.