CONICET | Buscador de Institutos y Recursos Humanos

INVESTIGADORES

DORFMAN Damian

datos académicos

artículos

libros

capítulos de libros

congresos y reuniones científicas

Título:

Retinal neuroprotection against acute retinal ischemia induced by isolated visual stimulation in adult rats

Autor/es:

MILNE, GEORGIA A; DIEGUEZ, HERNÁN H; GONZÁLEZ FLEITAS, MARÍA F; ARANDA, MARCOS L; IAQUINANDI, AGUSTINA; SANDE PABLO H; CHIANELLI, MÓNICA S; KELLER SARMIENTO, MARÍA I; ROSENSTEIN, RUTH E; DORFMAN, DAMIÁN

Lugar:

Buenos Aires

Reunión:

Congreso; Reunión Conjunta de Sociedades Biomédicas, LVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2017

Resumen:

Enrichedenvironment (EE) is a complex combination of inanimate and social interaction.EE boosts exploratory conduct, voluntary physical exercise, sensorial andcognitive functions, and social interaction. We have previously shown that EEinduced neuroprotection against unilateral retinal ischemia in adult rats. However,the relative contribution of each component to the effects of EE is stillcontroversial. Our aim was to dissect the individual contributions of the EErepertoire components in its protection against retinal ischemia. Adult male Wistar rats were subjected to unilateralor bilateral retinal ischemia and afterwards housed in stardard environment(SE), EE, social environment (SoE), novelty environment (NE), or visualenvironment (VE). SE consisted in stardard laboratory cages housing 2animals/cage. EE consisted in big cages (100 x 50 x 82 cm) housing 6animals/cage with a variety of objects, tunnels and running wheels dailyrelocated and weekly replaced. SoE and NE consisted in SE housing 5animals/cage, and EE objects respectively. VE consisted in SE cages surroundedby monitors showing black/white contrast (or grey patterns as controls) duringthe 12 h light phase. An antagonist for brain-derived neurotrophic factor(BDNF) Trk-b receptor (ANA-12) was administered to animals with unilateralretinal ischemia exposed to black/white contrast patterns. Retinal function(electroretinography) and histology were analyzed 3 weeks after ischemia. Retinalischemia induced functional and histological alterations (p<0.01), whichwere not protected by SoE or NE. The retinal protection achieved by EE againstunilateral ischemia (p<0.01) was abolished when ischemia was inducedbilaterally, regardless of motor activity. The exposure to VE achieved retinalprotection against unilateral ischemia (p<0.01), which was prevented byANA-12. These results suggest that visual stimuli, likely in a BDNF dependentmanner, could account for the retinal protection induced by EE.

enviar mensaje