INVESTIGADORES
DORFMAN Damian
congresos y reuniones científicas
Título:
Exposure to an enriched environment as a novel strategy to prevent acute ischemic damage of the visual pathway in adult rats
Autor/es:
DORFMAN D; FERNANDEZ DC; ROSENSTEIN RE
Lugar:
Fort Lauderdale
Reunión:
Congreso; The Association for Research in Vision and Ophthalmology; 2012
Institución organizadora:
Association For Research In Vision And Ophthalmology (Arvo)
Resumen:
Purpose
Ischemia is a key
component of several retinal diseases that are leading causes of irreversible
blindness. At present, there are no effective strategies to prevent or reverse
retinal ischemic damage. Recent evidences indicate that the exposure to an
enriched environment (EE) affects the functioning of the visual system. The EE
constitutes a strategy that boosts exploratory, visual, and cognitive
activities as well as social interaction and voluntary physical exercise. In
this context, the aim of the present work was to analyze the effect of exposure
to an EE in an acute retinal ischemia model.
Methods
Adult male Wistar
rats were exposed to a standard environment (SE) or EE 3 weeks before and 2
weeks after retinal ischemia. EE consisted of big cages housing 6 animals and
containing several food hoppers, wheels and different objects repositioned
once/day and fully substituted once/week. Retinal ischemia was induced by
increasing intraocular pressure to 120 mm Hg for 40 min. Retinal function
(electroretinography, ERG), and histology were analyzed at 7 and 14 days
post-ischemia. Anterograde transport from the retina to the superior colliculus
(SC) was examined after an intravitreal injection of cholera toxin β-subunit,
and retinal and SC glial fibrillary acidic protein (GFAP) levels were assessed
by immunohistochemistry.
Results
In control
animals, ischemia induced a significant decrease in ERG a- and b- wave
amplitude, whereas the exposure to EE reduced these alterations. In animals
exposed to SE, ischemia provoked retinal ganglion cell (RCG) loss which was
decreased by exposure to EE. Moreover, ischemia induced a significant increase
in GFAP levels in the retina and the SC, and a deficit in the anterograde
transport which were reduced in animals exposed to EE.
Conclusions
These results
suggest that the exposure to an EE could become a new strategy for retinal
ischemia treatment.